Magnesium oxide nanoparticles coated with glucose can silenceimportant genes of Leishmania major at sub-toxic concentrations

tThe aim of this study was to investigate the effect of magnesium oxide nanoparticles (MgO NPs) andMgO NPs coated with glucose (MONPCG) on Leishmania (L) major. First, the promastigotes of L. majorwere separately incubated with serial concentrations of MgO NPs and MONPCG for 24, 48, and 72 hat 37◦C. Then, the cell viability of promastigotes was evaluated by MTT assay. On the other hand, therelative expression of Cpb and GP63 genes was detected by quantitative-real time PCR. Based on results,the increase of concentration, both MgO NPs and MONPCG, and incubation time led to decrease of cellviability. Moreover, the expression of Cpb and GP63 genes was decreased with increase of concentrationof MgO NPs and MONPCG. Also, the increase of incubation time led to decrease of their expression in MgONPs treated promastogotes. But, in case of MONPCG treated promastogotes, the increase of incubationtime did not change the expression of Cpb and GP63. Interestingly, MONPCG could silence Cpb and GP63genes better than MgO NPs. Note, the capability was also seen at sub-toxic concentrations of MONPCG

Magnesium oxide nanoparticlesGlucoseLeishmania majorSub-toxic