A mononuclear oxido-vanadium(IV) complex, [VO(L)2], has been prepared from the reaction of
dibenzoylmethane (HL) and VO(acac)2 in a 2 : 1 molar ratio, and fully characterized using elemental
analyses, molar conductivity, FT-IR, and electronic spectroscopy. The structure of this compound was
also confirmed by single crystal X-ray diffraction. It was found that in the title complex, the metal
coordination geometry is described as a distorted square pyramid. DNA binding activities of this
complex were investigated using electronic absorption titration, competitivefluorescence titration and
cyclic voltammetry studies. The obtained results showed groove binding of the complex to salmon
sperm DNA accompanied with a partial insertion of the ligand between the base stacks of the DNA with
a binding constant of 2.310
3
M
1
. In addition, the interaction of the complex with bovine serum
albumin (BSA) was studied using electronic absorption andfluorescence spectroscopies at different
temperatures indicating a good affinity of the complex for BSA. These experimental results were
confirmed by the results of molecular docking. Finally, the in vitro cytotoxicity properties of the
synthesized complex against MCF-7, HPG-2 and HT-29 cell lines were evaluated and compared with
those of the ligand (HL). It was found that complexation improved the anticancer activity significantly.
IC50values for the V(IV) complex against MCF-7, HPG-2 and HT-29 cell lines were obtained as 7.8, 13.5
and 16.1mM, respectively
کلید واژگان :
MTT assy, V(IV) complex, Dna binding, BSA interaction, molecular docking
