tThe phenolic compounds of Zataria multiflora extract, were identified by HPLC analysis.Gallic acid, catechin, caffeic acid, and quercetin were found to be the major phenolic com-pounds. Eighty healthy laboratory Balb/C mice were infected intraperitoneally by injectionof 1500 viable protoscoleces and were divided into prevention (40 mice) and therapeutic(40 mice) groups. To prove the preventive effect of Z. multiflora extract on development ofhydatid cyst, the 40 infected animals were allocated into three treatment groups includ-ing Z. multiflora (4 g/l in drinking water for 8 months), albendazole (150 mg/kg BW/day for10 days) and untreated (control) group. To estimate the therapeutic effect of Z. multifloraextract on the hydatid cyst, after 8 months of infection, the infected mice were allocatedinto three experimental treatment groups including Z. multiflora (8 g/l in drinking waterfor 30 days), albendazole (300 mg/kg BW/day for 20 days) and untreated (control) group.At the end of the treatment period, all mice were euthanized and necropsied, the hydatidcysts were carefully removed, weighed and their size were recorded. Weight and size ofthe hydatid cysts significantly decreased (p < 0.05) upon the treatment with Z. multifloraextract in both prevention and therapeutic groups. The germinal layer of the hydatid cystsrecovered from the treated mice, either from the prevention or therapeutic group, werecompletely damaged at ultrastructural level by scanning electron microscopy.
کلید واژگان :Preventive,Therapeutic, Methanolic extract, Zataria multiflora, Hydatid cys, tIn vivo
ارزش ریالی : 600000 ریال
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