Currently, cell microencapsulation (CM) is a promising technology for a wide range of areas of interest in
cellular and molecular biology. This technique can be used for large-scale cell culture, cell implantation,
and cell-based gene therapy. Although several materials have been used for CM, alginate is considered to
be quite effective. Alginate bead-containing cells can be prepared by extruding a cell–alginate mixture into
a calcium chloride solution. It is possible to easily create calcium alginate (Ca-alginate) beads with a
syringe. However, beads that are generated by syringes are not suitable for CM due to their large size
(.1000 mm). Researchers have proposed a number of solutions for this problem. The use of certain
equipment is an inseparable part of these solutions. This study demonstrated that small size (,400 mm)
Ca-alginate beads can successfully be generated by modifying the syringe method. In this study, this
method was successfully employed for CM and embryoid body (EB) formation in alginate.
