Investigating experimental Pathogenicity of white spot syndrome virus in Litopenaeus vannamei using PCR method

Background and objective: Providing healthy food and required for increasing population of the country, that their health is important for future physical and mental health, Including responsibilities that it should be considered by wise strategy. Among these, marines have multiple capabilities for preventing diseases and providing health and in comparison with other proteins are more completely nutrients. In this study pathogenicity of virus of white spot disease that is the cause of the death and destruction of shrimps, in the Litopenaeus vannamei have been studied experimentally. Materials and methods: In this research, 200 Litopenaeus vannamei weighing approximately 10-12 gr were randomly selected ,and Infected animals being tested, and by using diagnostic kits IQ2000 PCR have been confirmed. From the frozen body of Indian white shrimps that previously have shown symptoms of white spot disease, with using Nested Polymerase chain reaction (Nested-PCR) method, the virus existence was proven, that was used as virus source, and during a day in several meals along with food was given to Litopenaeus vannamei. After 3 days, clinical symptoms including : rapid separation of the cuticle from the epidermis, empty stomach and intestines, yellow and brittle of hepatopancreas, and red moving limbs was observed. Samples of tissues from treatment groups for study PCR and next steps were selected. Results: samples with Nested-PCR method, according to instructions of diagnostic special kit of white spot disease, multiple reaction steps passed and Finally, by ultraviolet rays were observed and recorded, of which 90 samples, in 62 cases positive reactions were observed

Experimental Pathogenicity, White spot syndrome virus, Litopenaeus vannamei, PCR.