Iron overload is a major problem in Beta thalassemia patient due to regular blood transfusions. Regularly iron chelation is more recommended after 10-20 blood transfusions practice or when serum ferritin is more than 1000 ng /ml. The aim of present study was evaluated of chelating activity of Allium porrum and Medicago sativa. Materials and Methods: The total phenol, antioxidant activity and chelation capacity of plant extracts were estimated. For in vitro study of iron chelating, plant extracts were expose with serum thalassemic for 2h. Results: Total phenolic content and antioxidant activity in Hydro- alcoholic and phosphate buffer extracts were reported with maximum and minimum contents respectively. Iron content in thalassemia and control serums was estimated 181.6 ± 18.1 and 87 ± 17.3 respectively. A significant difference was found between iron concentrations in absence and presence extracts p<0.001. Iron levels in normal and thalassemia significantly reduced when exposed with plants extracts especially in Medicago sativa in phosphate buffer p<0.001. Iron concentration in serums control (86.9) after mixed with Medicago sativa phosphate buffer extract 66.2 ± 4.07 and Allium porrum phosphate buffer extract 75.2 ± 6.8 were reported. Conclusion: Medicago sativa and allium porrum with high iron chelating activity was a good plant chelator for in vitro study. These extracts can be a candidate for treatment of iron overload.
کلید واژگان :β- Thalassemia major,iron chelating, Iron overload,Medicago sativa, Allium porrum
ارزش ریالی : 600000 ریال
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