In this work,a novelthermo-responsive Fe3O4/poly(methylmethacrylate-b-N- isopropylacrylamide-b-acrylic acid) magnetic composite nanospherewas synthesized for anticancer drug delivery applications. For this propose, the poly(methylmethacrylate-b-N- isopropylacrylamide-b-acrylicacid) [poly(MMA-b-NIPAAm-b-AAc)] was synthesizedviareversible addition-fragmentation transfer (RAFT)method.The physic- chemical characterization of the Fe3O4/poly(MMA-b-NIPAAm-b-AAc) magnetic composite nanosphere was investigated by FTIR, HNMRspectroscopies and GPC,FESEM, XRD, VSM,andDLS. The thermo-sensitivity of the Fe3O4/P(MMA-b-NIPAAm-b-AAc) magnetic composite nanospherewas confirmed via DLS at 40 °C. DOX encapsulation efficiency was calculated to be 98.2%. The effect of temperature and pH on release behaviors of stimuli responsive DOX-loadedFe3O4/P(MMA-b-NIPAAm-b-AAc)] magnetic composite nanospherewere investigated. The release rate at pH 7.4, 5.4 and 4 (T=37° C) was reached about 24.4wt% ,42.4 wt% and 57.5 wt%, after 4-5 day. The release rate improved at tumor simulatedenvironment (t:40 °C and pH≤5.4).The cytotoxic effects of the magnetic composite nanospherewere appraised by MTT assay and the resultsindicated that novel developed smart nanocomposite here was nontoxic to MCF-7 cells and can be applied as anti-cancer drug delivery system.Also, the results ofthe Cellular uptake of MCF7 cells treated with rhodamine labelled DOX-loaded nanocarrier for 2 hrs have indicated that DOX can be applied as cytotoxic agent and targeting ligand.
کلید واژگان ::RAFT,magnetic composite nanosphere, drug delivery, tumor targeting, stimuli responsive polymer
ارزش ریالی : 1200000 ریال
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