Horseradish peroxidase (HRP) can be readily extracted from horseradish roots. In this research, the application of acid blue-25 (AB-25) dye removal by low purity horseradish peroxidase extracted from horseradish root (crude HRP) was investigated. Parameters, such as amount of HRP, contact time, pH, H2O2 concentration, AB-25 concentration and temperature during enzyme-mediated dye degradation and detoxification, were studied. Results showed that decolorization of AB-25 at 75 min contact time, pH 7and 0.8 mM/L H2O2 for 1 U/ml crude and pure HRP were approximately 80 and 94%, respectively. Also, gradually adding of H2O2 and enzyme increased the turnover of the HRP compared to control. Polyethylene glycol (PEG) in various concentrations did not significantly affect the reduction of HRP inactivation. Toxicity test with Daphnia magna showed a reduction in toxicity after the enzymatic treatment. To meet the optimized conditions in the process, the ratios of dye/ enzyme and H2O2/dye, and sequences of the addition of reactants, should be considered.
کلید واژگان :Horseradish peroxidase; azo dye; enzymatic treatment; crude enzyme
ارزش ریالی : 600000 ریال
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