CDH1 AND FGFR2 GENE POLYMORPHISMS IN PATIENTS WITH BREAST CANCER

Through various studies, a strong heritable background has been related to breast cancer. Approximate cases who exhibit a family history of the disease are 15%. Mutations in genes starkly in BRCA1 and BRCA2 make people subject to an autosomal dominant inherited cancer possibility. Cadherin-1 also known as E-cadherin (CD324) is a protein that in humans is encoded by the CDH1 gene which is a tumor suppressor gene. Fibroblast growth factor receptor 2 (FGFR2) is a protein that is encoded by the FGFR2 gene, is a receptor for fibroblast growth factor. CDH1 and FGFR2 genes play a role in carcinogenesis. It has already been demonstrated that polymorphisms in CDH1 and FGFR2 genes is related to various malignant disorders. The aim of present study was to reveal the affection possibility towards breast cancer in relation to SNPs of CDH1 and FGFR2 genes. Peripheral blood of 100 breast cancer contracted women and 150 healthy controls were collected. Afterward, genomic DNA content of the PBMCs was extracted. PCR-RFLP was done and in order to detection of polymorphisms, AflIII and AciI restriction enzymes treated with PCR product of CDH1 and FGFR2 genes, respectively. Restriction enzymes could digest the DNA at selected SNPs. According to statistical analysis, SNPs were not significantly frequent in patients with breast cancer in comparison to healthy controls. Nucleotide changes comparison between patients and healthy controls depicted that there was not SNP frequency significantly in patients of North West of Iran.

SNP, CDH1, FGFR2, Breast Cancer