In this study, oregano essential oil (OEO) has been encapsulated in chitosan nanoparticles by a two-step
method, i.e., oil-in-water emulsion and ionic gelation of chitosan with sodium tripolyphosphate (TPP).
The success of OEO encapsulation was confirmed by Fourier transform infrared (FT-IR) spectroscopy,
UV–vis spectrophotometry, thermogravimetric analysis (TGA) and X-ray diffraction (XRD) techniques.
The obtained nanoparticles exhibited a regular distribution and spherical shape with size range of
40–80 nm as observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). As
determined by TGA technique, the encapsulation efficiency (EE) and loading capacity (LC) of OEO-loaded
chitosan nanoparticles were about 21–47% and 3–8%, respectively, when the initial OEO content was
0.1–0.8 g/g chitosan. In vitro release studies showed an initial burst effect and followed by a slow drug
release.
کلید واژگان :
Chitosan Encapsulation In vitro release Nanoparticles Oregano essential oil
