Abstract In this study, peptide fragments were produced
from goat milk casein by proteolytic enzymes trypsin and ficin
and combination of both enzymes, and their antimicrobial
activity was investigated. After enzymatic treatments, antimicrobial
activity against both Gram-positive and Gramnegative
bacteria was enhanced. The hydrolysates were
passed through ultrafiltration. The obtained hydrolysate
by ficin (molecular weight (MW) <3 kDa) showed the
highest antimicrobial activity and was selected for further
purification by reversed-phase high-performance
liquid chromatography (RP-HPLC). Twenty-seven peptide
fractions were separated, and their antimicrobial activities
were evaluated. The results showed that one of the
fraction numbers (14) possessed the highest activity against
Escherichia coli and Bacillus cereus. The results suggest that
bioactive peptides obtained by digestion of goat milk caseins
with ficin could be exploited to as natural antimicrobial agents
in pharmaceutical industries
کلید واژگان :
Goat milk . Caseins . Bioactive peptide . Antimicrobial . E. coli . B. cereus
