The aim of this study was to determine the effects of deoxynivalenol (DON) contaminated diet on performance, immune system, gut morphology and jejunal gene expression in broiler chickens. Eighty-one-day old chicks were randomly allotted into two treatments with 4 replicates (10 birds in each replication). Experimental diets were the control diet (maize-soybean meal) and an experimentally contaminated diet with 10 mg/kg DON. The results indicated that DON-challenged birds had decreased (P < 0.05) average feed intake (AFI) during starter period as compared to control group. Also, average daily gain (ADG), AFI and feed conversion ratio (FCR) were not affected (P > 0.05) by inclusion of DON contaminated diet during the whole experimental period. Dietary addition of DON to the basal diet caused Fabricius bursa relative weight reduction, while increased the abdominal fat and serum triglyceride (TG) concentration (P < 0.05). Dietary DON feeding caused an enhancement (P < 0.05) in the blood aspartate aminotransferase (AST) and gamma glutamytransferase (GGT) contents. Moreover, DON decreased the serum total protein (TP) and albumin (ALB) concentrations. Inclusion of DON in diet reduced (P < 0.05) the white blood cell (WBC) count, lymphocyte number and antibody titer against Newcastle disease virus, but increased (P < 0.05) the blood heterophil count. The DON consumption also diminished (P < 0.05) the villus height, villus to crypt ratio, mucosa thickness and villus surface area in the duodenum. Mucin-2 expression was decreased (P < 0.05) by DON consumption, but toll-like receptor-4 (TLR-4) and claudin-5 (CLDN-5) expressions were not affected (P > 0.05) by dietary treatments. In conclusion, although DON could not influence the performance attributes in broiler chickens, it adversely affected the immune response, muc-2 gene expressions in the jejunum and gut morphology, enhanced the liver enzyme indices and lessened the blood protein contents.
کلید واژگان :Claudin-5 Deoxynivalenol Enzyme activity Gut morphology Mucin-2 White blood cell
ارزش ریالی : 600000 ریال
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