Molecular detection of Bovine Leukemia Virus (BLV) in the frozen semen samples of bulls used for artificial insemination in Iran

Bovine Leukemia virus (BLV) is a causative agent of bovine leukosis responsible for lymphoproliferative disorders in cattle. This virus was identified in reproductive tissues and fluids such as fresh and frozen semen samples, embryos and uterine fluids of animal origin. The aim of this study was to detect the BLV in the frozen semen samples of Iranian bulls used for artificial insemination using PCR. A total of 45 frozen Semen samples were obtained from 45 bulls (from semen bank), used for artificial insemination centers, Iran. DNA was extracted and 385 bp fragment of the gag gene of the virus was visualized in a 1% agarose gel electrophoresis. Viral DNA was detected in 9 of the 45 (20%) frozen semen samples of bull’s that were used for artificial insemination in Iran. The results of this study demonstrate the high prevalence of BLV DNA in frozen semen samples of bulls in Iran. According to this finding it is clear that testing, segregation and culling programs can be effective in the control and prevent the bovine leukosis disease caused by BLV. Furthermore, examination of cattle semen samples that are used for artificial insemination is necessary to prevent the spread of BLV in this region.

Artificial Insemination; Bovine leukemia virus; Bulls; PCR, semen