ISOLATION AND IDENTIFICATION AEROBIC THERMOPHILIC BACTERIA FROM HOT SPRING RAMSAR

Members of the genus Bacillus are considered the most frequently isolated thermophilic aerobes from terrestrial and marine hot water environments. Thermophilic microorganisms are found to be potential and good alternative source of thermostable enzymes. Terrestrial hot springs support thermophilic prokaryotic communities. In this study, the samples were enriched and cultivated in the modified medium named mBTM. SO, phenotyping and genotyping characteristics of isolated bacteria have been shown by morphological, biochemical and molecular tests. According to the results of this study, isolated bacteria are belong to the thermophilic genera of Bacillus. according to a study of diversity is wide aerobic bacilli. 16S rDNA sequence analysis showed that have higher similarity with B. licheniformis. Existence of B. licheniformis in thermal area was also reported in many other studies. However, this is study that demonstrated that B. licheniformis population is common bacterial species present in hot spring Ramsar, Ramsar, IRAN.

Thermophiles, mBTM, Hot Spring, 16S rDNA Sequence