Manganese is an essential metal in human that
functions in many enzymes. In contrast excessive exposure to Mn results in neurotoxicity. Accumulation of manganese damages central nervous system and causes Parkinson disease like
syndrome called manganism. Mn neurotoxicity
has been suggested to involve an imbalance in
catecholamine neurotransmitters. It hypothesized
that Mn can obstruct catecholamine synthesis
pathway by inhibition of Tyrosine hydroxylase.
Previous studies demonstrated that chronic and
acute dose of Mn has different possible effects
on catecholamine synthesis. It’s assumed that
an acute dose of manganese can kill dopaminergic cells. Therefore, we focused the effect of
Mn in catecholamine concentration on the rat’s
brain by MnCl2 injection intraperitoneally and
analyzed their brains after the time interval. This
study used different acute doses in short time
courses and different chronic doses at different
exposing times to investigate which of them (exposing dose or time) is more important in Mn
toxic effect. Measurement of catecholamine concentration performed by fluorescence assay in
acidic and oxidant environment.
