Proteins and peptide are an important class of drugs for treatment of various medical disorders. Insulin is a peptide drug, which is usually administered parenterally for treatment of diabetes mellitus. Despite being effective in administration of therapeutics, administration by parenteral route is often limited by pain, needle phobia and poor patient compliance. Therefore, a great attention has been paid to investigations into an alternative approach for effective delivery of insulin. The oral route for administration of insulin in type 1 diabetic patients, for instance, is considered more comfortable and convenient, compared with the subcutaneous route. However, oral delivery system suffers from weaknesses such as enzymatic degradation and limited capabilities to cross biological barriers. Therefore, development of an efficient delivery vehicle for oral administration of protein/peptide drugs presents an interesting challenge. This study reports the development of a highly stable niosomal nanoparticle composed of Span 60/cholesterol and N-trimethyl chitosan (TMC). Insulin loaded niosomes were made by reversed-phase evaporation technique and TMC coating was successfully carried out by incubation of the niosomal suspensions with the TMC solution. The efficacy of niosomal nanoparticle in increasing insulin permeation across intestinal cell monolayers was investigated by Caco-2 cell. The prepared nanoparticles were analyzed by Zetasizer and TEM, and their biocompatibility was investigated by cell viability measurements. This formulation represented good function for opening the tight cell junctions and was able to facilitate insulin permeability through Caco-2 cell monolayer as intestinal membrane models. Further work is required to optimize this formulation with a view to maximizing its potential to facilitate oral delivery of insulin.
کلید واژگان :Insulin; Niosomal nanoparticles; Trimethyl chitosan, Permeation
ارزش ریالی : 300000 ریال
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