Evaluating the Protective Effect of Co-administrating of Vitamin C and Royal Jelly on Phenylhydrazine-induced Anemia’s Derangements on Sperm Quality, Serum Parameters and Histological Parameters of Testicular Tissue in Mice

Abstract Background: Reduction in blood circulation results in pathological alterations in testicular physiologic function and spermatogenesis. Moreover, free iron ions from RBC lyses and consequently enhancement of iron ions in testicles leads to oxidative stress and affects the functions of reproductive system Materials & Methods: Thirty two mature mice weighting 20-25gr were assigned into 4 groups including control (received 0.2mL saline normal. ip), control-sham (received phenylhidrazine (PHZ), 6 mg/100g , ip), PHZ+vitamin C (250mg/kg) and royal jelly (100mg/kg, orally) and royal jelly+Vitamin C. Following 35 days, the blood samples were obtained from hearth and the serum levels of total antioxidant capacity (TAC), malondialdehyde (MDA), Testosterone, LH, FSH, and Prolactine were evaluated. The sperm parameters including; count, motility and DNA damage, chromatin condensation were assessed as well. the tissue samples were dissected out. Following, tissue passaging, embedding, section preparation the slides were underwent to H&E staining and special staining. The capsular thickness, germinal epithelium height, TDI, RI. SPI, seminiferous tubules diameter, tubular distribution, mean average of primary spermatocytes, mean number of Leydig cells, mean number of active Sertoli cells, MNICs and mast cells number were assessed. Results: Observations revealed a significant reduction in evaluated parameters, which was improved/inhibited in royal jelly and vitamin C co-treated group. Conclusion: Taking together, it can be concluded that co-administrating vitamin C and royal jelly was able to inhibit the detrimental impact of hemolytic anemia in mice.

Anemia, Phenylhydrazine, Vitamin C, Royal Jelly, Mice