MOLECULAR STUDY OF EXTENDED-SPECTRUM BETA-LACTAMASE (TEM-1) GENE IN ESCHERICHIA COLI ISOLATES COLLECTED, FROM RAZI HOSPITAL IN MARAND

Background and Aim: Emergence of antibiotic resistance is one of problems in microbial infection control. These types of enzymes can lead to resistances against the third generation Cephalosporins and hydrolysis of Monobactams. This study was conducted to evaluate antibiotic sensitivity profiles and the precence of blaTEM gene in E. coli isolates collected from clinical specimens of Razi Hospital in Marand. Methods: 70 isolates Escerichia coli were detected by using conventional bacteriologic tests and then antibniotic sensitivity tests were performed according to Kirby-Bauer method. Confirmatory test was also performed by Combind disk test method. Finally blaTEM gene was detected by using PCR technique. Results: Of the 70 isolates of Escherichia coli, 18 (25.71%) isolates produced broad-spectrum beta-lactamases, of which 15 (21.42%) had beta-lactamase TEM_1 genes.High resistance of E. coli isolates to the third generation Cephalosporins underlines need for accurate sensitivity tests, also avoidance from inappropriate use of antibiotics. Conclusion: High resistance of E. coli isolates to the Beta-lactam antibiotics underlines need for accurate sensitivity tests, also avoidance from inappropriate use of antibiotics.

Extended-Spectrum Beta-Lactamases (ESBLs), blaTEM gene, Escherichia coli , Polymrase Chain Reaction (PCR)