Fetal Bovine Serum (FBS) case study, Advantages and disadvantages of using it

Background: The use of cell cultures in tissue engineering, stem cell technology and cellbased therapy has become very important, and suitable for growth and proper metabolic function of cell culture. FBS is a complex combination of biomolecules with different molecular weights and promoting growth and growth functions, carrier, stabilizing and detoxifying factors. Methods:In a cross-sectional study, we compared the effect of two type of FBS on the cell line of NB4, while other conditions of the culture medium were constant. Viability and survival of all cells were estimated. Results: After several cultures, the results showed that NB4 with the type I of FBS was unable to grow properly and did not replicate.In the presence of type IIFBS, the cells returned to their normal state and multiplied. Conclusion: In terms of cell biology, the FBS deficiencies are complementary to the culture medium. Serum types of each lot number have shown quantitative and qualitative changes in their composition, which have been remarkable and effect on cell growth in the absence of oxygen. Therefore, the serum groups should be screened for the necessary permanent factors in the medium.

FBS, Cell Culture, Culture Medium