non-toxicity, biodegradability, biocompatibility and immunity system induction. The chitosan nanoparticle
was prepared by adding vitamin C to tripolyphosphate (TPP) solution and then blending it stepwise
to the chitosan solution under stirring. Nanoparticle characterizations were analyzed by scanning electron
microscopy (SEM) and Zetasizer. The release profile of vitamin C into the gastrointestinal and serum
of rainbow trout (Oncorhynchus mykiss) was portrayed and its effect on the immunity system of the fish
was determined as well. The results showed that the chitosan nanoparticle had spherical shape; positive
charge with particle size of 185 nm and in vivo controlled release until 48 h. Lysozyme and complement
contents in the fish serum increased as long as they were provoked by the nanoparticle. This nanoparticle
is a promising technique to boost immunity system of fish.
کلید واژگان :
Chitosan
Nanoparticle
Vitamin C
Controlled release
Gastrointestinal tract
