Background: Toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all
species of mammals including man. Congenital toxoplasmosis is more important during pregnancy for the
first time. In this study we expressed and purified P43 Toxoplasma gondii tachyzoite and bradyzoite specific
surface antigen.
Methods: The recombinant pGEMEX-1 contained Toxoplasma P43 coding sequence was transformed
into E. coli and mass cultured in LB medium contained 100 μg/ml ampicillin at 37°C over night .The T7
promoter was induced by 1mM isopropyl-1-thio-ß-D-galactopyranoside (IPTG. Recombinant protein was
purified by affinity chromatography and confirmed by gel diffusion dot blot and western blot,-using
specific anti Toxoplasma antibodies.
Results: Recombinant plasmid was induced by IPTG and analyzed by SDS-PAGE. Recombinant protein
was confirmed by Western-blot and dot blot using anti human Toxoplasma antibody.
Conclusion: Recombinant Toxoplasma P43 was produced successfully.
