Background and aims: OCT4B1, as one of OCT4 variants, were expressed in cancer cell lines and tissues more than other variants. We showed that this variant have an important role in regulation of cellular pathways as well as apoptosis and stress (heat shock protein). The aim of this study was to determine the effects of OCT4B1 silencing on expressional profile of HSP40 gene family in three different human tumor cell lines. Material and methods: OCT4B1 expression were suppressed followed specific siRNA transfection in AGS (gastric adenocarcinoma), 5637 (bladder tumor) and U-87MG (brain tumor) cell lines with Lipofectamine reagent. The real-time PCR array technique was employed and the fold changes were calculated using RT2 Profiler PCR array data analysis software version 3.5. Results: Our results indicated that the expression profile of 36 studied genes from HSP40 family in studied tumor cell lines after OCT4B1 suppression showed approximately similar pattern of expression. Fifteen genes were down-regulated and tow genes (DNAJC11 and DNAJC5B) were up-regulated in all three studied tumor cell line (more than 2 folds) and other studied genes (19 genes) showed different expressional pattern (up or down-expression) based tumor cell lines. Conclusions: we suggest there is a direct correlation between OCT4B1 expression in tumor cell lines (and tissues) and HSP40 family gene expressions to escape from apoptosis and cancer expansion.
کلید واژگان :OCT4B1, HSP40 gene family, siRNA, tumor cell lines
ارزش ریالی : 600000 ریال
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