This research study compares the biodegradation of petroleum hydrocarbons by natural
attenuation, biostimulation and bioaugmentation methods by applying the Pseudomonas aeruginosa
and Bacillus cereus bacteria in hydrocarbon-polluted land area of 1000 square meters consists of
three distinct parts to compare the methods discussed above for 100 days. The petroleumcontaminated
percentage of this area (w / w) is 0.5%. The extraction and determination of petroleum
hydrocarbons percentage in contaminated soil was done by gravimetric method by liquid-liquid
extraction method by hexane, dichloromethane and chloroform solvents. The results showed that the
bioaugmentation technique has a higher ability to analyze the petroleum hydrocarbons contaminant
that reduced about 82.33% of this pollution in the period of 100 days and biostimulation and natural
attenuation methods decreased the hydrocarbon materials percentage to the rate of 40.32 and
12.11% respectively.
