Objective: To investigate the caspase-1dependent inflammatory pathway activity and interleukin-1β (IL 1β) secretion in murine macrophage cell lines J774G8infected with Leishmania major (L. major)using caspase-1activity assay and ELISA.
Methods: Novy-MacNeal-Nicolle biphasic medium was applied to produce promastigote form of L. major. Metacyclic promastigotes in the stationary phase were applied to infect macrophage. Caspase-1 activity and IL-1β secretion were assessed by the CPP32/caspase-1 fluorometric protease assay and ELISA IL-1β kits, respectively, with time intervals of 6, 18and 30h.
Results: Our study showed an increase in caspase-1activity and IL-1βsecretion in infected samples compared to non-infected macrophages. The highest increase in IL-1β production was observed after 6h of infection.
Conclusions: These results arise that the activation of inflammasome pathway could be one of the innate immunity pathways against L. major
