Infectious diseases caused by fluoroquinoloneresistant strains of Klebsiella pneumoniae is a con
siderable challenge in healthcare issues. Mutations in gyrAgene nucleotide sequences are the most important
subject in the field of bacterial antibioticresistance mechanisms. In this study 200 cases of Klebsiella pneu
moniaehave been studied. For this purpose, 80 partial sequences of gyrAbelonging to fluoroquinoloneresis
tant strains of Klebsiella pneumoniaeobtained from the National Center for Biotechnology Information
(NCBI) were analyzed via bioinformatic software and 120 ready cases from several reported studies directly
imported into this investigation. Results showed that, two codons of Serine83 and Aspartic acid87 have the
most frequent mutations including Ser83 →Phe (33%), Ser83 →Tyr (20%) and Asp87 →Asn (25%),
Asp87 →Ala (8%) that have high hydrophobicity in comparison with normal amino acids. According to the
results of this investigation, the highdistribution of hot spot mutations in gyrAgene sequences may lead to
critical problems in the field of antibiotic therapy. Thus, the use of bioinformatics and statistical analyses are
prompt approaches to perceive and control the probable drugresistant bacterial strains.
