This study describes a high-performance electrochemical aptasensorwhich isemployed to detect Ibuprofen (IBP)
as a painkiller drug by using a novel platformas an integrated sensing interface. In order to make the aptasensor,
the Den-QD bioconjugate was immobilized on the surface of a GC electrode and followed the Apt was incubated
on this surface. The incubation of the IBP on the aptasensor surface and the formation of the Apt/IBP complex, led
to a hindered electron transfer reaction on the sensing surface, which decreased the peak current of the redox
probe. Under the optimum condition, the assay had two dynamic ranges with a detection limit down to
333 fM. The developed aptasensor reliably detects IBP in a real sample. Our results demonstrated that the proposed
strategy has many advantages and the Den-QD bioconjugate may become a promising nanocomposite
for the electrochemical sensing applications.
