Changes in the serum concentrations of aspartic aminotransferase (AST), alanine aminotransferase (ALT), rhodanese and arginase were measured in dogs, sheep and cattle with hepatic necrosis induced by the oral administration of carbon tetrachloride. A new method for arginase assay was based on the determination of remaining arginine (after its conversion to urea and ornithine) by its reaction with p-nitrophenyl glyoxa! (PNPG). In all species studied the serum arginase increased 6-12h after liver damage, reached a peak value in 4.8 h and returned to normal thereafter. Rhodanese activity did not change in dogs but rose significantly in sheep and, to a lesser extent, in cattle. AST increased strikingly in sheep as compared with dogs and cattle and remained high for >5 days. In dogs ALT rose sharply and remained elevated for > 10 days. No change in ALT was seen in sheep or cattle. The determination ofarginase by a simple procedure such as the PNPO method, in conjunction with AST or ALT assay, may be of value in assessing the stage of liver necrosis.
کلید واژگان :Arginase, Aminotransferases Liver Necrosis, Rhodanese,
ارزش ریالی : 600000 ریال
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