Na/K ATPase plays an important role in many cell functions in different organisms by generating an electrochemical gradient across the plasma membrane. Hence, phylogenetic and decision trees were used to study the evolutionary process and determine the effective attributes for classification Na/K ATPase α-subunit in Arthropoda and Nematoda as the most important phyla of the Ecdysozoa. Phylogenetic analysis identified that there were two distinct groups, the common (group I) and the scarce group (group II). Nematoda and Arthropoda sequences were completely separated from each other indicated that the separation of α-NKA subunit was according to divergence of phyla. The sequences from insects were also completely separated from other Arthropoda. Two lineages of ATPase α-subunit, which was referred to as group I and II, were distinguished that all members of group I had the assembling β-subunit motif, but the sequences fell in group II had not. The results also revealed that other important motifs for the function of this pump were absent in the sequences fell in the group II. All of these results suggest different functions for two groups of ATPase α-subunit. The results showed the phylogenetic tree based on the sequences of insects was the same as phylogenetic tree based on the phylum. The results of attribute weighting showed the ratio of Asp/Cys was the most important protein attribute in the separation of ATPase α-subunit in Arthropoda and Nematoda. In decision tree, this attribute was also played a critical role in the classification of ATPase α-subunit. In general, different amino acid ratios and the frequency of different dipeptides were the most attributes in most weighting algorithm models, and cysteine was the most important amino acid.
کلید واژگان :Keywords: α-NKA subunit Asp/Cys ratio Decision tree Insects
ارزش ریالی : 600000 ریال
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