Sequencing and Sequence Analysis of Growth Hormone Type 1 (GH1) Gene Analogue in the Salmo Trutta Caspius

The Growth hormone gene is more important in the regulator of metabolism, osmoregulation, reproduction and skeletal growth in Livestock. Specially, in the Salmonids. Furthermore, growth hormone gene can be used as the fusion of phylogenetics, population genetics and allows assessment of the impact of historical events on the current relationships among different populations. Growth hormone gene in the Atlantic Salmon (Salmo salar) has full length of almost 3.5 kb at different reports in the Gene Bank. For studying of growth hormone gene in the Salmo trutta caspius, was extracted DNA genomics from bloods and the muscles of salmons, in related to, first we designed three pairs of the primers from the full length of the growth hormone gene. These primers can be synthesis different fragments which contain five introns and six exons in the full length of GH1. After doing PCR, PCR products were purified using the Gel extraction SPIN-50 (RKT33) kit, then sequence products, analyzed by the NCBI Network system. Sequence fragments were shown there is a high homology with other GH gene salmons in the exon and intron fragments. Furthermore, a complete sequence of GH gene in the Salmo trutta caspius deposited in the GeneBank, accession number, JN241634.1. That is including 2041 by a length. In this research we had two aims, first, the study of amount variation in the between salmo trutta caspius species with Atlantic salmon and second aim, the study amount of variation Salmo trutta caspius with other salmons regarding to the growth hormone gene.

Growth hormone gene, Salmo trutta caspius