Reducing Sugar Assessment on Three Medicinal Plants with Inoculation of Arbuscular Mycorrhiza and Growth Regulators

The interactive potential benefits of inoculation with arbuscularmycorrhizal fungi (AMF) Glomus fasciculatum (Thax. Sensu Gerd.) Gerd, and Trappe to three medicinal plants such as Nirgandi (Vitex negundo L.), Henna (Lawsonia inermis L.) and Copper leaf (Acalypha wilkesiana L.), with IBA in the presence of (0, 1000, 15000 and 2000 mg L-1) conducted in earthen pots in factorial based completely randomized design with four replications and the plants were uprooted periodically 90 days. Leaf samples for each harvest were analyzed for reducing sugar concentration. The results showed remarkably high reducing sugars content in Acalife plant at IBA concentration of 2000, 1000 and 1500 mg L-1, 111.56, 98.88 and 98.61 mg L-1, respectively. The lowest reducing sugars content was in the extract of Henna plant (at 2000 ppm) with 31.17 mg L-1. The highest rate of reduced sugar in three medicine plants Vitex negundo, Lawsonia inermis and Acalypha wilkesiana was related to Acalypha wilkesiana plant that had higher reduced sugar compared to two other plants. With increasing in IBA hormone concentration up to 1500 mg L-1 the rate of reduced sugar significantly increased in Vitex negundo plant, but it was decreased in 2000 mg L-1 concentration. 1500 mg L-1 treatment with the highest rate of reduced sugar in Vitex negundo plant was significantly different from control treatment. The highest rate of reduced sugar in 1000, 1500 and 2000 mg L-1 IBA belonged to Acalypha wilkesiana plant.

Arbuscular Mycorrhizal Fungi, Indol-3 Butyric Acid, Reducing Sugar