As a new science, genomics explores the sequencing of genomes in specific organisms. One type of genomics is comparative genomics which can be regarded as a valuable tool for develop molecular resources for crops and identify major genes in plants. Increasingly, the accessibility of sequence data for genomes of multiple species allows us to compute their evolutionary pathway on a genome-wide basis. This in turn allows a better understanding of the homologous relationships between genes. The present study has made an attempt to identify and compare conserved areas for tufm gene in common bean (Goli and AND1007), chickpea (Bivanij and ILC482) and pea (Flagman and native Gorgan) genomes using STS markers. Evaluation of protected areas in these plants showed that the differences between cultivars the tufm gene have emerged. For this purpose after amplification and observation of polymorphism, the isolated fragments of each sample were sequenced. The NCBI database and DNAStar software provided valuable information on the genomes of the crops including the conserved areas. The highest and lowest similarity was belonging to Bivanij and ILC-482, and Flagman and Goli, respectively. ILC-482 and Flagman has highest difference, and ILC-482 and Bivanij has lowest difference among all cultivars. This could be as the result of the mutations in evolution process. Except for chickpea, it was also revealed that intraspecies sequences are far greater than interspecies.
کلید واژگان :comparative genomics, genomic conservation, legumes, STS marker, tufm.
ارزش ریالی : 600000 ریال
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