Rapid Regeneration of DracocephalumKotschyi Boiss. from Nodal Explants

The Dracocephalum KotschyiL., a wild-growing flowering plant belonging to the family Lamiaceae is best known for the essential oils common to many members of the family. The present study showed the procedure for propagation of D. kotschyiusing nodal segments from in vitro germinated plants. A high frequency and rapid regeneration protocol was growing and developing from nodal explants of D. kotschyion the medium MS containing 2mg/L BAP plus 0.5mg/L NAA. Histochemical analysis showed a direct induction of more teratological protuberances that arise around the cut end of the explants. After hardening, the rooted plants were transferred to the greenhouse condition where they normally grew, matured and flowered with a survival rate of 90-95%. We concluded that the present protocol can be efficiently used for mass propagation of D. kotschyi.

Dracocephalum Kotschyi; Explant; Medicinal; Lamiaceae