In vitroAchievement of Strawberry Roots Formation Using ag (NO3)

In the present study In vitroeffective micropropagation protocol was studied in Fragaria, using axillary bud explants. The effects of addition silver nitrate Ag (NO3) and Plant growth regulation on in vitro shoot formation were investigated. The addition of silver nitrate at concentrations of 1-5 mg/l to the shoot induction medium the MS supplemented with 0.5 mg/l BAP plus KIN 0.2 mg/l induced multiple shoot formation with all used explants at mean rates of between 2 and 3 shoots per explant. The best concentration of BAP for buds induction was 0.5 mg/l plus KIN 0.2 mg/l and Ag(NO3) 4 mg/l. The maximum elongation and number of roots per explant were obtained in same medium with MS combined with 0.5 mg/l BAP with KIN 0.2 mg/l and Ag(NO ) 4 mg/l. In addition using this medium constriction enhanced of roots formation medium, all of them 3 the seedlings had safe and excellent rooting. The rooted plants were transferred to the greenhouse condition where they normally grew, matured and flowered with a survival rate of 100%. It is concluded that the present protocol can be efficiently used for in vitromass propagation of strawberry

Ag (NO3) Axillary bud Fragaria Multiple shoot Rosaceae