Lemon balm (Melissa officinalis L.) is an important medicinal plant in the family of Lamiaceae. The purpose of the current study was to test the applicability of leaf protein patterns for assessment of genetic diversity of 25 accessions of Melissa officinalis. The leaf protein of 45 days seedling was extracted using HEPES/KOH buffer and separated by SDS-PAGE technique. The cluster analysis was performed based on protein bands using weighted pair group method arithmetic averages (WPGMA) of DARwin 5 software and PCoA. The results indicated a total of 18 bands ranged from 10 to 150kDa of which 59.09 percentage were polymorphic. The maximum number of the bands (16) was observed in Germany accession, while Italy and Mashhad accessions had the minimum number of bands (10). The cluster analysis of 25 accessions based on the protein data produced five clusters. The first cluster involved 4 accessions, the second cluster contained six accessions, the third cluster contained three accessions, the fourth cluster contained five accessions and fifth cluster contained seven accessions. Overall, the outcomes of the present study indicated a high genetic diversity among the M. officinalis accessions. As a result of this investigation, protein markers would be a useful tool for assessment of genetic diversity among Lemon balm genotypes, and the findings suggest that the plants belonging to different clusters can be used for intraspecific hybridization to generate useful recombinants in the segregating generations for breeding of M. officinalis.
کلید واژگان :Genetic diversity; Melissa officinalis; Protein pattern; SDS-PAGE
ارزش ریالی : 300000 ریال
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