Phytochemical studies and their bioactivities from Beilschmiedia Palembanica (Miq.) Kosterm

(+)-kunstlerone (1) as a neolignan, together with nine known alkaloids: (+)-norboldine (2), isocaryachine (3), pseudocodamine (4), milonine (5), sebiferine (6) and (-)-salutarine (7), noratherosperminine (8), 10-O-methyl hernovine (9), atherosperminine (10), and the amide was cinnamide (11), were isolated from the leaves of Beilschmiedia Palembanica (Miq.) Kosterm. The structures were established through various spectroscopic methods notably 1D- and 2D-NMR, UV, IR and LCMS-IT-TOF. (+)- Kunstlerone (1) showed a strong antioxidant activity, with an SC50 of 20.0 μg/mL Additionally, the effect of compounds 1, 2 and 3 were evaluated on A549, PC-3, A375, HT-29 and WRL-68 cell lines. Kunstlerone 1 showed moderate cytotoxicity against various cancer cell lines such as A549, PC-3, A375, HT-29 and WRL-68, respectively with EC50 values of 28.02, 26.78, 33.78, 33.65 and 16.46 μg/mL. The crude methanol extract showed antigrowth activity against S. pyogenes II and B. cereus, with MICs of 256 μg/mL. The compounds kunstlerone (1), isocaryachine (3) and noratherosperminine (8) showed complete inhibition against P. shigelloides, with MIC ≤ 60 μg/mL compare to ampicillin, as a positive control, which showed antigrowth activity against P. shigelloides at MIC 10 μg/mL.

Keywords: Beilschmiedia Palembanica, alkaloid, neolignan, lauraceae, antioxidant, anticancer