Staphylococcus pasteuri strain RA3T a gram positive, non-spore forming, non-motile, catalase positive was isolated from surface soil. The identification of the bacterium was confirmed by 16S rDNA phylogeny and biochemical tests. TatD is a cytoplasmic protein and exhibits a magnesium-dependent DNase activity. The TatD family has no involvement in protein export by the Tat system. A TatD DNase gene was isolated and cloned from Staphylococcus pasteuri strain RA3T genome. Nucleotide sequence analysis revealed 774 nucleotides in length encoding a protein of 257 amino acid residues. The TatD gene showed a high similarity to homologs belonging to Staphylococcus warneri SG1. The deduced polypeptide sequence harbors a typical catalytic site, HHRPEDHRHFSSGEDPLN and its calculated molecular mass and its predicted isoelectric point are 29656.7 Da and 5.05, respectively. The deduced amino acid sequence showed a high degree of similarity to TatD DNase isoforms from Staphylococcus genus and other sources. Three dimensional predictions of TatD confirmed the active site and its theoretical functions as DNase.
کلید واژگان :Soil bacteria, TatD DNase, Staphylococcus
ارزش ریالی : 300000 ریال
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