A new method to detect extracellular deoxyribonuclease enzyme in bacteria cell

DNase enzyme hydrolyzes the bonds between nucleotides and almost detects by DNA agar medium test in bacteria. In this paper, we present possibility of a new method for detecting extracellular DNase. The new test procedure was investigated and optimized. Two type of DNA Escherichia coli ATCC 25922 chromosome and pET-21a(+) plasmid were applied and time treatments were also examined. Cation treatments like Cu2+, Mg2+, Ca2+ and Zn2+ as cofactor were applied. This new DNase test can determine the existence of extracellular DNase enzymes and approximately recognize the enzyme type. The method is easy to carries out and requires no expensive reagents and equipment. The best time for genomic DNA and pET-21a(+) plasmid digestion were 24 and 6 h, respectively. Extracellular DNase extraction was set up to bacteria cells. Results showed independence of test from all cations.

Extracellular enzyme, DNase, bacteria