The HIV-1 genome encodes 9 genes, comprising gag, pol, env, tat, rev, vif, vpu, vpr and nef. The gag
gene encodes a polyprotein precursor whose name, Pr55Gag, is based on its molecular weight. Here,
we are describing Gag-polyprotein, starting with its sequence, homology and phylogenetics.
Additionally, consisting motifs and structural properties of the Gag-polyprotein were considered.
Following their compartmentalization, we studied their genomics, transcriptomics, and proteomics
data. We have concluded that CA or p24 peptide of Gag polyprotein has conserved domain that can be
utilized for diagnosis of HIV infection. In addition, Gag polyprotein is absolutely essential for
packaging the viral particles; if siRNAs or shRNAs could be designed for conserved domains of p24
then virus replication cycle would be interrupted.
