Effect of unilateral blunt testicular trauma on mononuclear immune cells infiltration in the contralateral testis in mice

Background: In children, blunt testicular trauma is often a sports-related injury and can result in contusions, rupture, and, in rare cases, torsion of the testicle. Though, effects of unilateral blunt testicular trauma (UBTT) on the contralateral testis are still a matter of debate. This study was thus designed to examine the effect of UBTT on contralateral testicular histology in pre-pubertal mice. Methods: Male NMRI mice aged 20 days were randomized into three equal groups. Following anesthesia with ketamine (5 mg/100 g body weight; IP), abdomens of group I (control) were sham operated without disturbing either testis. In group II (UBTT), the abdomen was opened and the left testis was placed on a sterile firm surface and 5 g sterile weight was dropped on to the testis from a height of 10 cm.in group III sildenafil citrate was administrated till 7 days after trauma.mice were At around 70 days of age the left testis of all animals was harvested and microscopic evaluation of MNICs infiltration determined by using the unbiased counting frame. Results: Histological studies revealed that UBBT in mice led to marked elevation of MNICs infiltration in the contralateral testis. Conclusion: The results suggest that UBTT produces an immunological response in the contralateral testis, which may have the potential to adversely affect fertility in cases of unilateral testicular trauma.

Blunt testicular trauma, Mononuclear immune cell, Testis, Mouse