Evaluation of Sperm Fertility Quality in Phenyl Hydrazine-Induced Hemolytic Anemia Impacts on Mice

Background: Anemia and consequently low oxygen can cause changes in the function and structure of the testis and spermatogenesis, Iron released from red blood cells, followed by the increased oxidative stress, which may cause tissue iron. This study was designed to minimize the deleterious effects of hypoxia on the genitals. Materials and Methods: Eighteen mature male mice were randomly divided into 3 groups. In order to induce experimental anemia, PHZ was administrated at dose of 8 mg/kg, ip and then it was continued as 6 mg/kg, every 48 hours, ip in all groups. The test groups subdivided into non-treated PHZ-received, royal jellyreceived (100 mg/kg, orally) and vitamin C-received (250 mg/kg, ip). After 35 days Semen samples were collected from epididymis for IVF study with healthy oocytes. Results: The results of this study showed that anemia-related hypoxia resulted in a drastic reduction in quantity fertilized oocytes, The two-cell stage oocytes and blastocyst, and increase the number of stopped Oocytes. Conclusion: These results indicate the effect of the anemiarelated hypoxia on sperm Fertility quality. These results indicate the effects of oxidative stress caused by anemia (hypoxia) induced By phenylhydrazine on the in-vitro fertilization quality of mouse sperm.

Phenylhydrazine, Hemolytic Anemia, IVF