Evaluation the effect of PUFAs oral administration on level of P53 gene in gastric cancer undergoing chemotherapy

Background and Aim : In recent years experimental studies indicate the fact that a diet rich in fish oil and unsaturated fatty acids may be used as a cure or protection against different types of cancers. Gastric cancer is the most common type of cancer in the world and statistically has the second rank as the cause of deaths among the different types of cancer. Respond to the treatment is differentin the gastric cancer patients, and some of the tumors areresistant to this kind of chemotherapy. Hence, one of the obstacles in succession of chemotherapy is increasing the resistant to this type of chemotherapy. Thus, our purpose from development of this study is to understand whether it is possible to use PUFAs fatty acids along with the common chemotherapy drugs such as platinums, as a factor for activation of P53 in the patients with gastric adenocarcinoma. Methods : This study is a Clinical Trial that the target group is patients with gastric cancer who were recognized for the first time and were cured under chemotherapy. Thirty-four patients were chosen in two groups of 17 subjects. Three biopsy samples from tumor were taken these patients. At first group, chemotherapy started with only Cis-Platin, and in the second group, chemotherapy started with PUFAs supplement in the scale of 3600 mg daily and in length of 3 courses. Once again, biopsy sampled from these patients. Levels of mRNA expression of P-53 samples were measured in biopsy samplesby Real-Time PCR technique. Results : The means of P53 Gene in gastric tumor after 3 course chemotherapy in 2 groups of study , shows increase , but this increase was more greater in second group and it is meaningful from the statistic point of view ( p= 0.012 ) . Conclusion : Results of this study show that PUFAs effect of medication and also usual drugs of active P53 Gene, which were used for controlling of gastric cancer, can beneficial.

Gastric Cancer, PUFAs, P53 Gene, Chemoresistance