MOLECULAR STUDY OF THE PREVALENCE OF CTX-M1, CTX-M2, CTX-M3 IN PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL SAMPLES IN TABRIZ TOWN, IRAN

Background and Aim:Pseudomonas aeruginosa is the most common pathogen causing nosocomial infections. One of the reasons for the occurrence of drug resistance in the Pseudomonas aeroginosa strains is the production of the enzymes of Extended-Spectrum Beta-lactamases. The aim of this study was to determine the antibiotic resistance and the frequency of the enzymes of Extended-Spectrum Beta- lactamases (CTX-M1,CTX-M2,CTX-M3) in the Pseudomonas aeroginosa strains isolated in hospitals of Tabriz Town. Methods:The Pseudomonas aeroginosa strains were collected from different samples of patients admitted to hospitals and medical centers in Tabriz Town during a year from 26th December, 2014 to 25th December 2015 and after identifying the phenotypic and genotypic identity (Sr RNA 16) and performing antibiogram test and double-disk synergy method, the phonotype of ESBLs in the bacterium of Pseudomonas aeruginosa was evaluated, then the bacterial DNA was extracted and studied and evaluated by PCR method and using special primers for the frequency of genes Results:110 Pseudomonas aeruginosa strains were identified from 1500 isolates collected from the hospitals and medical centers in Tabriz Town that in them, the highest resistant was related to the antibiotics of Amikacin (81.81%),Nalidixic acid (89.09%) and Ceftriaxone (75.45%). The highest gene frequencies of ESBLs are related to the genes of CTX-M1 (27.27%), CTX-M2 (23.63%) and CTX-M3 (9.09%), respectively. Conclusion:The gene studied in this research were all on the chromosomes of the bacterium of Pseudomonas aeruginosa. So, investigating the genes of ESBL like CTX-M1 which has maximum frequency in the isolates of Pseudomonas aeruginosa seems necessary.

ESBL , gene of CTX-M, Pseudomonas aeruginosa