The Evaluation of miR-191 Downregulation on Erythroid Lineage Differentiation of Murine Embryonic Stem Cells

Introduction: Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos. Their importance to modern biology and medicine derives from two unique characteristics that distinguish them from all other organ-specific stem cells identified to date. Non-coding microRNAs (miRNA) play an important role in development timing. They might influence hematopoietic lineage differentiation. Recent studies have demonstrated that downregulation of miR-191 have key roles in erythropoiesis. Materials and Methods: Murine embryonic stem cells (mESCs) were infected with lentiviruses containing miR-451. Erythroid differentiation was evaluated using quantitative RT-PCR for hemoglobin chains genes and flowcytometery for erythroid surface antigens. CFU assay was also performed at days 10th , 14th and 21th. Results: MiR-191 down -regulation, could induce α, β and γ-globin expression in mESCs and have strong correlation with appearance of CD235a and Ter-119 markers in these cells. Moreover, miR-191 downregulation increases erythroid cells in CFU assay. Conclusions: Our results demonstrate that miR-191 dow -regulation strongly induces erythroid differentiation and maturation of mESCs. In the other hands our results provide that down-regulation of miR-191 have the potential role in erythroid differentiation of mESCs .

miRNAs, miR-191, Murine embryonic stem cells (mESCs), Erythropoiesis.