Here, we report the first genome sequence of a
new virulent phage of Microbacterium oxydans, termed
vB_MoxS-ISF9, which was isolated from sewage. Trans-
mission electron microscopy showed that the isolated
phage, which has a hexagonal head of about 80 nm in
diameter and a long non-contractile tail of about 240 nm,
belongs to the family Siphoviridae. The vB_MoxS-ISF9
DNA was completely sequenced and found to be 59,254 bp
in length, with a G?C content of 62.76 % and 120 putative
open reading frames (ORFs). The predicted protein pro-
ducts of the ORFs were identified, and their sequences
were analyzed. In a comparison with all available phage
genomes, vB_MoxS-ISF9 did not show any significant
similarity to other previously reported bacteriophages. To
the beast of our knowledge, this is the first report of the
isolation and complete genomic sequencing of a virulent
phage against a member of the genus Microbacterium.
