TISSUE ENGINEERING AND HISTOLOGY OF OSTRICH TENDON

Tendons are soft connective tissues, which connect muscle to bone forming a musculotendinous unit, whose primary function is to transmit tensile loads generated by muscles to move and enhance joints stability. The main goal of tissue engineering design and create a structure similar to the natural structure of a tissue in vivo for repair tissue damages. Tissue transplantation, there are always problems, including a shortage of suitable tissue and immunological rejection of grafted tissue is in tissue engineering is one of the newest methods to reduce the immune response to transplanted tissue, is tissue decellularization methods. After fixation and routine tissue process, to evaluate the structure of tissue H&E stained, collagen quality assessment xenograft Picrosirius red stained , review and tracking the decellularization, detected nuclei trace and any DNA remaining 4 ' , 6'diamino-2-phenylindole-2HCl (DAPI) staining was used. Evaluation of properties of decellularization tendon structure and comparison with ostrich tendons found this xenograft is the appropriate for graft. Results by Mann-Whitney (P≤0.05) were performed with SPSS software. Compared with ostrich tendons and decellularization tendon, the tissue integrity is somewhat reduced cohesion between collagen fibers were observed in some places a few miles away. Quality collagen stained by Picrosirius red that there is no difference between them.In assessing the amount of decellularization following DAPI staining completely cell-free of the three-dimensional scaffold and remains free of nuclear DNA.

Xenograft, Achilles tendon, scaffold, decellular, Ostrich