Objective: Acute myeloid leukemia has the highest mortality among leukemias and the factors cause this leukemia, is miRNAs. Generally, disrupted of the expression of miRNAs can play a role in changing the regulation of proteins in vital and important cell pathways. On the other hand, components that affect the miRNA expression have a high importance therapeutic effect. Therefore, we study the effect of sulforaphane on acute myeloid leukemia cell lines and the expression pattern of miR-155 and miR-181a. Material and Methods: In this study, 4 classes of HL-60, U937, KG1 and NB4 were used for the treatment. The doses used for the treatment of the cells were 15, 30, 45 and 60 μM of sulforaphane in 24 and 48 hours. After treatment, miR-155 and miR-181a expression were evaluated. In order to determine the apoptosis of the cells, a flow cytometry was used at that time. Results: Our findings suggest that sulforaphane reduces the number of live cells and increases the mortality rate of myeloid rats. (P<0.05). The use of this substance caused a significant reduction in the expression of both miRNA in the cells of patients group (P<0.05). Reduced expression of miR-155 can be positively related to the factors and proteins involved in the cellular circulation. The reduced level of miR-181a will probably affect the regulatory genes in the process of differentiation and proliferation of myeloid. Apoptosis assays also showed that the highest apoptosis has occurred in 60 μM of sulforaphane in KG1 cells with 46.60% rate of apoptosis. Conclusion: The results indicate that sulforaphane increases the death rate of AML cells and decreases the expression of miR-155 and miR-181a. In this study, the number of live cells decreased. Anyway, in future studies, it is better to performance intervention inhuman in order to determine the clinical effects of sulforaphane.
کلید واژگان :Myeloid leukemia, miR-155, miR-181a, sulforaphane
ارزش ریالی : 600000 ریال
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