Purpose Cancer stem cells (CSCs) have been suggested to represent the main cause of tumour progression, metastasis and drug resistance. Therefore, these cells can be an appro- priate target to improve cancer treatment. Methods A novel biodegradable brush copolymeric micelle was synthesized by the ring-opening polymerization (ROP) and reversible addition-fragmentation chain transfer (RAFT) polymerization. The obtainedmicelle was used for co-delivery of the anticancer drug docetaxel (DTX) and Chrysin (CHS) as an adjuvant on the CSCs originated from Human colon ad- enocarcinoma cell line. Cancer stem cells were enriched by MACS technique and characterized by flow cytometry anal- ysis against CD133 marker. Results Data demonstrated that the micelles harbouring DTX@CHS had potential to reduce cancer stem cell viability compared to free DTX@CHS, single-drug formulations and the control group ( < 0.05). The combination effect of DTX and CHS formulated inmicelle was synergistic in CSCs (CI < 1). The reactive oxygen species content was shown to increase after cell treatment with DTX@CHS loaded on micelles ( <0.05). DTX@CHS-micelles inhibited cancer stem cell migration rate ( < 0.05), indicating an impaired metastasis activity. Conclusion In conclusion, the synthesized DOX@CHS-mi- celles can be applied in the introduction of anticancer agents to resistant cancer population by further investigations
کلید واژگان :brush copolymer . cancer stemcell . combination therapy . docetaxel . thermosensitivemicelle
ارزش ریالی : 600000 ریال
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