The Determination of ω-Fatty Acids (PUFAs) Effect on caspase-8 gene expression of stomach in patients infected with H. pylori

Background: Drug-resistant strains and poor treatment response is the main reason for the failure in Helicobacter pylori (H. pylori) eradication. Unsaturated fatty acids with more than one double bond (PUFAs) have inhibitory effect on bacterial growth. Therefore, the aim of this study is to investigate the effect of Omega fatty acids on caspase-8 gene expression in H. pylori infected patients with dyspeptic symptoms. Methods: In this double blind clinical trial study in which 34 H. pylori infected patients with dyspeptic symptoms were randomly divided into two groups of 17 patients, the control group receiving standard triple therapy (including amoxicillin, clarithromycin and omeprazole) and the intervention group receiving standard therapy with omega-3, omega-6 and omega-9 fatty acids for two weeks. After treatment, Gene expression levels of Caspase-8 enzymes as well as the semi-quantitative of these proteins were studied with PCR Real-time method and frozen section and immunohistochemistry, respectively. Results: In the case group compared with the control group, a significant increasing in the expression of caspase-8 genes was observed (p= 0.002). The protein level of active caspase-8 enzymes was significantly increased in the case group compared with the control group (0.004). Conclusion: The Omega fatty acids along with standard triple therapy lead induce apoptosis is stomach.

H. Pylori, Unsaturated Fatty Acids, Caspase-8, Stomach