Nystatin Candida Albicans drug loading by Electrospinning: Preparation and Characterization

The objective of this study was to prepare a suitable form of nanofiber for Nystatin using polymers Eudragit HPMC (Hydroxy Prapyl Methyl Cellulose) K4M and RS PO and to evaluate the effect of some variables on the characteristics of resulted electrospunnanofibers. Electrospinning process was used for the preparation of nanofibers. Several solutions the combinations of HPMC (Hydroxy Prapyl Methyl Cellulose) K4M, Eudragit RS PO and Ibuprofen in different solvents and various ratios were prepared. The spinning solutions were loaded in 10 mL syringes. The feeding rate was fixed by a syringe pump at 2.0 mL/h and the use a high voltage supply at range 10-18 kV was applied for electrospinning. Electrospunnanofibers were collected and evaluated by scanning electron microscopy, differential scanning calorimetry and FTIR for possible interaction between materials used in nanofibers. The effect of solvent and viscosity on the characteristics of nanofibers also was investigated. Fiber structure was successful using a solvent ethanol and the combinition of Eudragit HPMC (K4M) and (RS PO). Increase in viscosity of ethanolic solutions of Eudragit (RS PO) followed by addition of HPMC (K4M) in the solution led to preparation of smooth fibers with larger diameters and less amounts of beads. DSC analysis of fibers accepted that Nystatin is evenly distributed in the nanofibers in an amorphous state. FTIR analysis did not indicate significant relationship between drug and polymer. Evidence has shown that drug-loaded HPMC (K4M) and Eudragit (RS PO) nanofibers could be prepared by exact selection a range of variables such as type of solvent, drug: polymer ratio and solution viscosity and the optimized formulations can be useful for drug delivery.

Nystatin, Eudragit, Electrospinning, Nanofibers.