Macrophomina phaseolina is a plant pathogenic fungus which have a broad range of host such as soybean, bean, chickpea, pine and sesame. This fungus is a soil born pathogen that infects its hosts in primary growth stages like seedling growth. In this study, antifungal activity against Macrophomina phaseolina, was examined and inhibition percentage values was calculated. According to activity and broad spectrum of isolates, three isolates showed a high activity against charcoal rot and one of them had the highest activity. Some morphologically appearance suggested that these species belong to genus Streptomyces and phylogenetic studies using 16SrDNA confirmed it. Morphological changes such as hyphal branching and abnormal shape were observed in fungi grown on ISP2 medium. Hence, Streptomyces sp. BJ03 highly produced extracellular chitinase on colloidal chitin agar plates, it seems that this antifungal activity of this strain is related to production of extracellular chitinase. Therefore, the selected strain could be used for biocontrol of phytopathogenic fungi.
کلید واژگان :
Macrophomina phaseolina , Streptomyces sp. BJ03, biological control
